鈣蛋白酶2催化亞基

鈣蛋白酶2催化亞基
已知的結構
PDB	直系同源搜索: PDBe RCSB
PDBID列表
	1KFU、1KFX、2NQA
識別號
別名	CAPN2；, CANP2, CANPL2, CANPml, mCANP, calpain 2
外部ID	OMIM：114230 MGI：88264 HomoloGene：1326 GeneCards：CAPN2
基因位置（人類）
染色體	1號染色體
終止	223,776,018 bp
基因位置（小鼠）
染色體	小鼠1號染色體
終止	182,345,173 bp
RNA表達模式
	查閱更多表達數據
基因本體
分子功能	• calcium ion binding; • 半胱氨酸型肽酶活性; • 金屬離子結合; • cytoskeletal protein binding; • 肽酶活性; • 血漿蛋白結合; • protein heterodimerization activity; • 水解酶活性; • calcium-dependent cysteine-type endopeptidase activity; • 酶結合;
細胞組分	• 高爾基體; • 偽足; • 膜; • 粘着斑; • perinuclear endoplasmic reticulum; • 胞內; • 樹突; • 內質網; • cortical actin cytoskeleton; • 脂筏模型; • 染色質; • 溶酶體; • 外排體; • 細胞核; • 細胞質基質; • 細胞質; • 細胞膜; • mitochondrial intermembrane space; • external side of plasma membrane; • cell projection; • soma;
生物學過程	• response to hypoxia; • protein autoprocessing; • 蛋白酶解; • cellular response to amino acid stimulus; • myoblast fusion; • proteolysis involved in cellular protein catabolic process; • regulation of cytoskeleton organization; • blastocyst development; • extracellular matrix disassembly; • female pregnancy; • positive regulation of cardiac muscle cell apoptotic process; • regulation of interleukin-6 production; • cellular response to interferon-beta; • response to hydrogen peroxide; • behavioral response to pain; • cellular response to lipopolysaccharide; • positive regulation of neuron death; • positive regulation of myoblast fusion; • positive regulation of phosphatidylcholine biosynthetic process;
	Sources:Amigo / QuickGO
直系同源
	824
	12334
	ENSG00000162909
	ENSMUSG00000026509
	P17655
	O08529
	NM_001146068; NM_001748
	NM_009794
	NP_001139540; NP_001739
	NP_033924
	維基數據
檢視/編輯人類	檢視/編輯小鼠

鈣蛋白酶2催化亞基（英語：Calpain-2 catalytic subunit）是人類中由CAPN2基因編碼的蛋白質。^[5]^[6]

功能

鈣蛋白酶是鈣激活的中性蛋白酶，是非溶酶體的細胞內半胱氨酸蛋白酶。哺乳動物鈣蛋白酶包括普遍存在的胃特異性和肌肉特異性蛋白質。普遍存在的酶由異二聚體組成，這些異二聚體具有與常見的小調節亞基相關的獨特的大催化亞基。該基因編碼普遍存在的酶鈣蛋白酶2的大亞基。已報道5' UTR中存在多個異質轉錄起始位點。^[7]

相互作用

CAPN2已被證明與Bcl-2產生相互作用。^[8]

參考資料

^ ^1.0 ^1.1 ^1.2 GRCh38: Ensembl release 89: ENSG00000162909 - Ensembl, May 2017
^ ^2.0 ^2.1 ^2.2 GRCm38: Ensembl release 89: ENSMUSG00000026509 - Ensembl, May 2017
^ Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K. Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease. Biochemistry. 1988, 27 (21): 8122–8. PMID 2852952. doi:10.1021/bi00421a022.
^ Hata A, Ohno S, Akita Y, Suzuki K. Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease. J. Biol. Chem. May 1989, 264 (11): 6404–11. PMID 2539381. doi:10.1016/S0021-9258(18)83364-6 .
^ Entrez Gene: CAPN2 calpain 2, (m/II) large subunit.
^ Gil-Parrado S, Fernández-Montalván A, Assfalg-Machleidt I, Popp O, Bestvater F, Holloschi A, Knoch TA, Auerswald EA, Welsh K, Reed JC, Fritz H, Fuentes-Prior P, Spiess E, Salvesen GS, Machleidt W. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J. Biol. Chem. Jul 2002, 277 (30): 27217–26. PMID 12000759. doi:10.1074/jbc.M202945200 .

延伸閱讀

Suzuki K, Sorimachi H, Yoshizawa T, Kinbara K, Ishiura S. Calpain: novel family members, activation, and physiologic function. Biol. Chem. Hoppe-Seyler. 1995, 376 (9): 523–9. PMID 8561910. doi:10.1515/bchm3.1995.376.9.523.
Cohen GM. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326 (Pt 1): 1–16. PMC 1218630 . PMID 9337844. doi:10.1042/bj3260001.
Reverter D, Sorimachi H, Bode W. The structure of calcium-free human m-calpain: implications for calcium activation and function. Trends Cardiovasc. Med. 2001, 11 (6): 222–9. PMID 11673052. doi:10.1016/S1050-1738(01)00112-8.
Kopp S. Reproducibility of response to a questionnaire on symptoms of masticatory dysfunction. Community Dent Oral Epidemiol. 1976, 4 (5): 205–9. PMID 1067155. doi:10.1111/j.1600-0528.1976.tb00985.x.
Adachi Y, Kitahara-Ozawa A, Sugamura K, Lee WJ, Yodoi J, Maki M, Murachi T, Hatanaka M. Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I. J. Biol. Chem. 1992, 267 (27): 19373–8. PMID 1527057. doi:10.1016/S0021-9258(18)41785-1 .
Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K. Four genes for the calpain family locate on four distinct human chromosomes. Cytogenet. Cell Genet. 1990, 53 (4): 225–9. PMID 2209092. doi:10.1159/000132937.
Ishiguro H, Higashiyama S, Namikawa C, Kunimatsu M, Takano E, Tanaka K, Ohkubo I, Murachi T, Sasaki M. Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations. Biochemistry. 1987, 26 (10): 2863–70. PMID 3038169. doi:10.1021/bi00384a030.
Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 1996, 271 (43): 27099–106. PMID 8900201. doi:10.1074/jbc.271.43.27099 .
Corasaniti MT, Navarra M, Catani MV, Melino G, Nisticò G, Finazzi-Agrò A. NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain. Biochem. Biophys. Res. Commun. 1996, 229 (1): 299–304. PMID 8954122. doi:10.1006/bbrc.1996.1796.
Fujitani K, Kambayashi J, Sakon M, Ohmi SI, Kawashima S, Yukawa M, Yano Y, Miyoshi H, Ikeda M, Shinoki N, Monden M. Identification of mu-, m-calpains and calpastatin and capture of mu-calpain activation in endothelial cells. J. Cell. Biochem. 1997, 66 (2): 197–209. PMID 9213221. S2CID 85163404. doi:10.1002/(SICI)1097-4644(19970801)66:2<197::AID-JCB7>3.0.CO;2-L.
Rock MT, Brooks WH, Roszman TL. Calcium-dependent signaling pathways in T cells. Potential role of calpain, protein tyrosine phosphatase 1b, and p130Cas in integrin-mediated signaling events. J. Biol. Chem. 1997, 272 (52): 33377–83. PMID 9407132. doi:10.1074/jbc.272.52.33377 .
Ueyama H, Kumamoto T, Fujimoto S, Murakami T, Tsuda T. Expression of three calpain isoform genes in human skeletal muscles. J. Neurol. Sci. 1998, 155 (2): 163–9. PMID 9562261. S2CID 205898607. doi:10.1016/S0022-510X(97)00309-2.
Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W. The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc. Natl. Acad. Sci. U.S.A. 2000, 97 (2): 588–92. Bibcode:2000PNAS...97..588S. PMC 15374 . PMID 10639123. doi:10.1073/pnas.97.2.588 .
Masumoto H, Nakagawa K, Irie S, Sorimachi H, Suzuki K, Bourenkov GP, Bartunik H, Fernandez-Catalan C, Bode W, Strobl S. Crystallization and preliminary X-ray analysis of recombinant full-length human m-calpain. Acta Crystallogr. D. 2000, 56 (Pt 1): 73–5. Bibcode:2000AcCrD..56...73M. PMID 10666632. doi:10.1107/S0907444999013748.
Chua BT, Guo K, Li P. Direct cleavage by the calcium-activated protease calpain can lead to inactivation of caspases. J. Biol. Chem. 2000, 275 (7): 5131–5. PMID 10671558. doi:10.1074/jbc.275.7.5131 .
Lee MS, Kwon YT, Li M, Peng J, Friedlander RM, Tsai LH. Neurotoxicity induces cleavage of p35 to p25 by calpain. Nature. 2000, 405 (6784): 360–4. Bibcode:2000Natur.405..360L. PMID 10830966. S2CID 205006589. doi:10.1038/35012636.

外部連結

The MEROPS online database for peptidases and their inhibitors: C02.002 （頁面存檔備份，存於網際網路檔案館）
Human CAPN2 genome location and CAPN2 gene details page in the UCSC Genome Browser（英語：UCSC Genome Browser）.

[refGRCh38Ensembl-1] 1.0 ^1.1 ^1.2 GRCh38: Ensembl release 89: ENSG00000162909 - Ensembl, May 2017

[refGRCm38Ensembl-2] 2.0 ^2.1 ^2.2 GRCm38: Ensembl release 89: ENSMUSG00000026509 - Ensembl, May 2017

[3] Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid2852952-5] Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K. Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease. Biochemistry. 1988, 27 (21): 8122–8. PMID 2852952. doi:10.1021/bi00421a022.

[pmid2539381-6] Hata A, Ohno S, Akita Y, Suzuki K. Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease. J. Biol. Chem. May 1989, 264 (11): 6404–11. PMID 2539381. doi:10.1016/S0021-9258(18)83364-6 .

[entrez-7] Entrez Gene: CAPN2 calpain 2, (m/II) large subunit.

[pmid12000759-8] Gil-Parrado S, Fernández-Montalván A, Assfalg-Machleidt I, Popp O, Bestvater F, Holloschi A, Knoch TA, Auerswald EA, Welsh K, Reed JC, Fritz H, Fuentes-Prior P, Spiess E, Salvesen GS, Machleidt W. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J. Biol. Chem. Jul 2002, 277 (30): 27217–26. PMID 12000759. doi:10.1074/jbc.M202945200 .

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閱論編蛋白酶類：半胱氨酸蛋白酶類（EC 3.4.22)
胱天蛋白酶	胱天蛋白酶1 胱天蛋白酶2 胱天蛋白酶3 胱天蛋白酶4 胱天蛋白酶5 胱天蛋白酶6 胱天蛋白酶7 胱天蛋白酶8 胱天蛋白酶9 胱天蛋白酶10 胱天蛋白酶11 胱天蛋白酶12 胱天蛋白酶13 胱天蛋白酶14
來自果實	木瓜蛋白酶無花果蛋白酶（英語：Ficain）鳳梨蛋白酶獼猴桃蛋白酶
鈣蛋白酶	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2
組織蛋白酶	B C F H K L1/L2 O S T W Z
其它	梭菌蛋白酶癌性促凝物質分離酶（英語：Separase） Autophagin Cruzipain 3C樣蛋白酶
EC 1.1/2/3/4/5/6/7/8/9/10/11/12/13/14/15/16/17/18/19/20/21/22 · 2.1/2/3/4/5/6/7(2.7.10/11-12)/8/9 · 3.1/2/3/4(3.4.21/22/23/24)/5/6/7/8/9/10/11/12/13 · 4.1/2/3/4/5/6 · 5.1/2/3/4/5/99 · 6.1-3（英語：Template:Ligases CO CS and CN）/4/5-6